Journal: bioRxiv
Article Title: Enhanced Differentiation Potential of Pigmented Human Epidermal Equivalents
doi: 10.1101/2025.05.29.656859
Figure Lengend Snippet: (A) Schematic representing the general formation of PmtHEEs. ALI, cultured at the air-liquid interface. (B) Melanin distribution in PmtHEEs. Top-view gross morphology of a PmtHEE after 14 days of ALI culture within the 24-well culture insert system (left), visualized using a stereomicroscope at 8x magnification. Histological analysis (right) includes haematoxylin and eosin (H&E) staining (top right), which illustrates epidermal morphology, and Fontana–Masson staining (bottom right), highlighting melanin distribution. Black arrowheads indicate regions of pigment accumulation. Scale bar, 50 µm. (C) Immunofluorescence analysis of KRT10, KRT14, LOR, and S100B expression. Nuclei are counterstained with DAPI (blue). White arrowheads indicate the positions of melanocytes. Scale bar, 50 µm.
Article Snippet: Slides were mounted with Fluoromount G® mounting medium with DAPI (SouthernBiotech, Birmingham, AL, USA).
Techniques: Cell Culture, Staining, Immunofluorescence, Expressing